Journal: Cell reports

Article Title: Epigenetic Reprogramming of Cancer-Associated Fibroblasts Deregulates Glucose Metabolism and Facilitates Progression of Breast Cancer

doi: 10.1016/j.celrep.2020.107701

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The following primary antibodies were used: CK8 (Throma-1; the University of Iowa, 1:50), Collagen I (SouthernBiotech 1310-01; 1:200), Collagen III (SouthernBiotech 1330-01; 1:200), Laminin 1 (Sigma L9393; 1:200), Tenascin C (Abcam ab108930; 1:200), αSMA (Sigma F3777; 1:200), FSP1 (a gift from Dr. Eric Neilson, Vanderbilt Univ.

Techniques: Recombinant, Electron Microscopy, Plasmid Preparation, SYBR Green Assay, Immunodetection, Bicinchoninic Acid Protein Assay, Modification, Luciferase, Derivative Assay, Isolation, shRNA, Control

Journal: Hypertension

Article Title: Calpain-1 Regulation of Matrix Metalloproteinase 2 Activity in Vascular Smooth Muscle Cells Facilitates Age-Associated Aortic Wall Calcification and Fibrosis

doi: 10.1161/hypertensionaha.112.196840

Figure Lengend Snippet: Figure 3. Overexpression of calpain-1 induces collagen type I, II, and III and reduces elastin expression levels in vascular smooth muscle cells (VSMC). A, Representative Western blots of collagen I and collagen III in VSMC after infection by GFP or CANP1 adenoviruses for 48 hours. B, Photomicrographs (×400) of collagen II protein staining (brown color) from young (8-month) and old (30-month) rat aortic walls. Nuclei are counterstained with hematoxylin (blue color). L indicates lumen; M, media. C and D, Representative Western blots and average protein levels of collagen II (C) and elastin (D) in young VSMC infected with GFP or CANP1 for 48 hours. *P<0.05 compared with GFP-infected 8-month VSMC, n=3.

Article Snippet: Primary antibodies used for immunostaining (IS) and Western blotting (WB) Antibody Species Titer for IS Titer for WB Source -actin Mouse 1:10000 Sigma-Aldrich Co. LLC, St. Louis, MO calpain-1 Rabbit 1:100 1:2500 Sigma-Aldrich Co. LLC, St. Louis, MO collagen I Rabbit 1:1000 Rockland Antibodies & Assays, Gilbertsville, PA collagen II Rabbit 1:50 1:1000 Abcam, Cambridge, MA collagen III Rabbit 1:1000 Rockland Antibodies & Assays, Gilbertsville, PA elastin Rabbit 1:1000 Millipore, Billerica, MA MMP2 Mouse 1:50 1:1000 Millipore, Billerica, MA MT1MMP Rabbit 1:1000 Millipore, Billerica, MA osteonectin Rabbit 1:1000 Millipore, Billerica, MA osteoponin Rabbit 1:1000 Millipore, Billerica, MA Smad 2/3 Mouse 1:1000 Cell Signaling Technology, Inc, Danvers, MA TGF-1 Rabbit 1:500 Santa Cruz Biotechnology, Inc, Santa Cruz, CA TIMP2 Goat 1:500 R&D Systems, Inc. Minneapolis, MN 9 Figure S1: Interactions between calpain-1 and MMP2.

Techniques: Over Expression, Expressing, Western Blot, Infection, Staining

Journal: Hypertension

Article Title: Calpain-1 Regulation of Matrix Metalloproteinase 2 Activity in Vascular Smooth Muscle Cells Facilitates Age-Associated Aortic Wall Calcification and Fibrosis

doi: 10.1161/hypertensionaha.112.196840

Figure Lengend Snippet: Figure 6. Overexpression of calpain-1 regulates extracellular matrix remodeling, particularly fibrosis and vascular calcification in cultured carotid artery rings. Representative Western blot of tissue inhibitor of metalloprotease 2 (TIMP2), calpain-1, elastin, transforming growth factor-β1 (TGFβ1), (A) Smad 2/3, collagen I, II, III, (B) and osteopontin (OPN) and osteonectin (ON) (C) in cultured carotid artery rings isolated from 8-month-old rat after infection by GFP or CANP1 or TIMP2 adenoviruses for 48 hours.

Article Snippet: Primary antibodies used for immunostaining (IS) and Western blotting (WB) Antibody Species Titer for IS Titer for WB Source -actin Mouse 1:10000 Sigma-Aldrich Co. LLC, St. Louis, MO calpain-1 Rabbit 1:100 1:2500 Sigma-Aldrich Co. LLC, St. Louis, MO collagen I Rabbit 1:1000 Rockland Antibodies & Assays, Gilbertsville, PA collagen II Rabbit 1:50 1:1000 Abcam, Cambridge, MA collagen III Rabbit 1:1000 Rockland Antibodies & Assays, Gilbertsville, PA elastin Rabbit 1:1000 Millipore, Billerica, MA MMP2 Mouse 1:50 1:1000 Millipore, Billerica, MA MT1MMP Rabbit 1:1000 Millipore, Billerica, MA osteonectin Rabbit 1:1000 Millipore, Billerica, MA osteoponin Rabbit 1:1000 Millipore, Billerica, MA Smad 2/3 Mouse 1:1000 Cell Signaling Technology, Inc, Danvers, MA TGF-1 Rabbit 1:500 Santa Cruz Biotechnology, Inc, Santa Cruz, CA TIMP2 Goat 1:500 R&D Systems, Inc. Minneapolis, MN 9 Figure S1: Interactions between calpain-1 and MMP2.

Techniques: Over Expression, Cell Culture, Western Blot, Isolation, Infection

Journal: Photonics

Article Title: In Situ Structural Characterization of Cardiomyocyte Microenvironment by Multimodal STED Microscopy

doi: 10.3390/photonics11060533

Figure Lengend Snippet: Reconstructed models show the endocardium (in AF), cardiomyocytes (in AF), and interstitial matrix (in B-SHG) within papillary muscle cross-sectioned areas. Collagen type III surrounds individual cardiomyocytes and the cardiomyocyte bundle (top row). β1 integrin distributes on cardiomyocytes and endothelial cells (bottom row). Arrowheads highlight nuclei of squamous endothelial cells within the endocardium. Z-step, 0.5 μm. Stack depth, 13 μm (top row); 11.5 μm (bottom row).

Article Snippet: Sections were blocked in 2% ( w / v ) bovine serum albumin with 0.3% Triton X-100 at room temperature, then incubated with anti-laminin (ab11575, Abcam, Cambridge, UK), β1 integrin (ab179471, Abcam), collagen type III (600-401-105, Rockland, Philadelphia, USA), or collagen type IV (ab179471, Abcam) at 4 °C overnight.

Techniques: